Wide field Raman imaging using the integral field spectroscopy approach was used as a fast. one shot imaging method for the simultaneous collection of all spectra composing a Raman image. For the suppression of autofluorescence and background signals such as room light. shifted excitation Raman difference spectroscopy (SERDS) was applied to remove background artifacts in Raman spectra. https://www.hindigyanvishv.com/hot-save-Herbstonne-Black-Eyed-Susan-big-savings/